trpm8 antagonist  (MedChemExpress)

Journal: Scientific Reports

Article Title: Control of motor coordination by transient receptor potential melastatin 8 through γ-aminobutyric acidergic circuit modulation in the male mouse cerebellum

doi: 10.1038/s41598-025-98837-9

Figure Lengend Snippet: Characterization of transient receptor potential melastatin 8 (TRPM8) immunoreactivity in the mouse cerebellum. ( A ) Green fluorescent protein (GFP) expression in the cerebellum of TRPM8-GFP mice. ( B ) TRPM8 expression in the whole cerebellum using light sheet fluorescent microscopy. ( C ) Double labeling of TRPM8 (green) with calbindin, parvalbumin, vesicular GABA transporter (VGAT), vesicular glutamate transporter 2 (VGLUT2), γ-aminobutyric acid (GABA) A receptor (GABA A -R), hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 (HCN1), glial fibrillary acidic protein (GFAP), and ionized calcium-binding adaptor molecule 1 (Iba-1) (red) in the cerebellum of TRPM8-EGFP mice. Dotted squares indicate the magnified areas. Scale bars: 100 μm ( A ), 20 μm ( C ).

Article Snippet: Animals received an intraperitoneal or intracerebellar administration of the TRPM8 selective antagonist RQ-00203078 (MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Expressing, Microscopy, Labeling, Binding Assay

Journal: Scientific Reports

Article Title: Control of motor coordination by transient receptor potential melastatin 8 through γ-aminobutyric acidergic circuit modulation in the male mouse cerebellum

doi: 10.1038/s41598-025-98837-9

Figure Lengend Snippet: Effects of transient receptor potential melastatin 8 (TRPM8) deficiency on the rotarod, open field, and footprint tests. ( A ) Latency to fall off the rotarod in wild-type (WT) and TRPM8-deficient (TRPM8KO) mice. Data are presented as the mean ± standard error (SE) for 9–10 mice per group. * P < 0.05 compared with WT. ( B ) Distance traveled in the chamber, average movement speed (velocity), and percentage of time spent in the central and peripheral areas in WT and TRPM8KO mice. Data are presented as the mean ± SE for 10 mice per group. * P < 0.05 compared with WT. NS, not significant. ( C ) Quantification of footprints in terms of stride, sway, and stance in WT and TRPM8KO mice. Data are presented as the mean ± SE for 7 mice per group. NS, not significant.

Article Snippet: Animals received an intraperitoneal or intracerebellar administration of the TRPM8 selective antagonist RQ-00203078 (MedChemExpress, Monmouth Junction, NJ, USA).

Techniques:

Journal: Scientific Reports

Article Title: Control of motor coordination by transient receptor potential melastatin 8 through γ-aminobutyric acidergic circuit modulation in the male mouse cerebellum

doi: 10.1038/s41598-025-98837-9

Figure Lengend Snippet: Effects of transient receptor potential melastatin 8 (TRPM8) deficiency on cerebellar weight, structure, and γ-aminobutyric acid (GABA)-related factors. ( A ) Cerebellar weights in wild-type (WT) and TRPM8-deficient (TRPM8KO) mice. ( B ) Representative images of hematoxylin and eosin staining (left) and Nissl staining (right) in WT and TRPM8KO mice. ( C ) Quantification of the number and soma size of Purkinje cells (calbindin-positive cells), number of parvalbumin-positive cells, and number of vesicular GABA transporter (VGAT)-positive puncta in WT and TRPM8KO mice. ( D ) Representative western blotting images of glutamate decarboxylase (GAD)65 and GAD67 proteins from WT and TRPM8KO mice, and quantitative western blotting results. ( E ) Comparison of GABA levels in WT and TRPM8KO mice. Data are presented as the mean ± standard error for 5–7 mice. NS, not significant. Scale bars: 500 μm ( B ), 20 μm ( C ).

Article Snippet: Animals received an intraperitoneal or intracerebellar administration of the TRPM8 selective antagonist RQ-00203078 (MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Staining, Western Blot, Comparison

Journal: Scientific Reports

Article Title: Control of motor coordination by transient receptor potential melastatin 8 through γ-aminobutyric acidergic circuit modulation in the male mouse cerebellum

doi: 10.1038/s41598-025-98837-9

Figure Lengend Snippet: Effects of transient receptor potential melastatin 8 (TRPM8) antagonist (RQ-00203078) administration on motor coordination. ( A ) Latency to fall off the rotarod in mice that were intraperitoneally treated with vehicle (control) or RQ-00203078. Data are presented as the mean ± standard error (SE) for 8–10 mice per group. * P < 0.05 compared with vehicle. ( B ) Latency to fall off the rotarod in mice that were treated with vehicle or RQ-00203078 via intracerebellar administration. Data are presented as the mean ± SE for 8 mice per group. * P < 0.05 compared with vehicle. ( C ) Double labeling of TRPM8 with c-Fos and γ-aminobutyric acid (GABA) in the cerebellum of a TRPM8-EGFP mouse. Scale bars: 20 μm.

Article Snippet: Animals received an intraperitoneal or intracerebellar administration of the TRPM8 selective antagonist RQ-00203078 (MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Control, Labeling

Journal: Scientific Reports

Article Title: Control of motor coordination by transient receptor potential melastatin 8 through γ-aminobutyric acidergic circuit modulation in the male mouse cerebellum

doi: 10.1038/s41598-025-98837-9

Figure Lengend Snippet: Schematic diagram of how transient receptor potential melastatin 8 (TRPM8) channels on inhibitory γ-aminobutyric acid (GABA)ergic neurons may contribute to motor coordination by modulating synaptic transmission at Purkinje cell–interneuron synapses. This image was generated using the Motifolio illustration toolkit (Motifolio Inc., Ellicott City, MD, USA).

Article Snippet: Animals received an intraperitoneal or intracerebellar administration of the TRPM8 selective antagonist RQ-00203078 (MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Transmission Assay, Generated